High Performance Liquid Chromatography I: Fundamentals, Equipment, and Operation
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Who Should Attend
The course is intended to develop an understanding of the principles, practice and instrumentation of HPLC for those who have begun to use the equipment for analysis or for research. Some exposure to chromatography and other analytical equipment is necessary. No specific educational level is assumed. This course has proven to be beneficial to persons with varied interests in HPLC.
Description
This course will demonstrate the great flexibility of high performance liquid chromatography which results from the variety of different mechanisms of separation. Various experts from the field will discuss the different modes of chromatography: steric exclusion, adsorption and partition (normal and reversed phase), ion exchange, ion pairing and ion chromatography. Equipment will be described, and the techniques necessary to ensure continuous operation will be delineated. Both qualitative and quantitative analyses will be considered, as well as techniques for trace work. The course will conclude by considering systematic development of an HPLC separation.