Course offering number:1004-706
Course ID:
2400
The Do’s, Don’ts, and Whys of HPLC
Pre-recorded Course
AN ON-DEMAND ONLINE TRAINING FORMAT
Who Should Attend
This online training will be most useful for people with little or no experience with using HPLC (High Performance Liquid Chromatography). This course will benefit laboratory personnel in
many industries including: pharmaceutical – traditional and phytochemical, food, beverage, environmental, chemical, and personal products.
The potential job functions begin with new laboratory technicians to researchers with degrees in other disciplines who need to begin their own
HPLC work. This includes: organic synthesis, quality control, and methods developments chemists and various research personnel.
The departments in companies involved with the use of HPLC in research/development or quality control/quality assurance should insist their
new personnel attend if they are not being trained in-house.
Description
This basic HPLC course will benefit the new or recent users of this analytical tool. This 90-minute accredited training will include discussions of
theory, definitions, and typical practice and will illustrate what HPLC is all about. What is inside the 'skin' of the LC system will give an
understanding of its basic simplicity. The course is designed to give the new user confidence in his/her use of this invaluable analytical tool.
Module 1:
• Principle & basic theory of HPLC including
modes of
chromatography
• The chromatogram and nomenclature
• The vanDeemter plot and its significance
• Monitoring the LC separation
• Use of the resolution equation to aid in
optimizing the LC
separation
• Peak shape, deterioration of the separation
• HPLC instrumentation, solvent reservoirs,
pumps
• Mobile phase - isocratic and gradient
operation
Module 2:
• Correct connections throughout the LC
system
• Injectors, laminar flow in empty tubing,
autoinjectors
• HPLC hardware, sizes, flow adjustments
with different
diameter column
• Detectors used in HPLC - the usual and
the specific
• Calibration curves for quantitative analysis
• Column packings, silica gel development,
bonding
possibilities
• Bonding terminology - endcapping, base
deactivated, polar
modified
• Solvent considerations, HPLC grade water,
degassing
mobile phases
Module 3:
• Reversed phase mode, USP column
classification
• RP column selection, other RP bonded
phases to consider
• Retention differences with % carbon
loading and RP chain
length
• Comparing different C18 phases
• Controlling pH and why, solvent choices for
optimization
• Ion pairing to retain compounds with strong
ionization
• Caution with buffer gradient separations
• LC columns out of the box, and their
storage
• Application help from the web and texts
Questions and Answer Session